Book ID: 93100
Murhammer, David W. (ed.)
Baculovirus and Insect Cell Expression Protocols. Second edition. 2007. (Methods in Molecular Biology, Volume 388). 3 col. illustr. 58 b/w illustr. XIV, 470 p. gr8vo. Hardcover.
This second edition is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production ofbaculovirus insecticides at both laboratory and large scale aredescribed in the kind of step-by-step format that the Methods in Molecular Biology series helped standardize. Includes sections on usingmodified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-ffree medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed.