Book ID: 101234
Sucher, Nikolaus, James Hennell and Maria C. Carles (eds.)
Plant DNA Fingerprinting. Methods and Protocols. 2012. (Methods in Molecular Biology, Vol. 862). illus. 230 p. gr8vo. Hardcover.
Molecular cloning and DNA-based analysis have becomepart of every molecular life science laboratory. The rapid adoption of DNA-based techniques has been facilitated by the introduction of the polymerase chain reaction (PCR), which has made cloning and characterization of DNA quick and relatively simple. PCR is virtually part of every variation of the plethora of approaches used for DNA fingerprinting today. The book aims to bring together the different currently available genome-based techniques into one repository. This volume contains detailed protocols for the preparation of plant genomic DNA, fingerprinting of plants for the detection of intra-species variations, the use of DNA barcoding, as well as methods for the bioinformatic analysis of data. Also included are several discussions on the broader issues of genome-based approaches in order to provide a sound understanding of the principles of these methods.