Plant Epigenetics. Methods and Protocols. 2010. (Methods in Molecular Biology, Vol. 631). illus. X, 273 p. gr8vo. Hardcover.

The past fifteen years have witnessed major advances in epigenetics, one of the most popular and quickly evolving fields of modern science. In the book researchers explore the most recent developments, examining in great detail the contribution of epigenetic regulation to cell function in plants. Chapters include a variety of protocols for studying the function of small non-coding RNAs, DNA methylation, and histone modifications in plants, often in different degrees of complexity. This volume describes bioinformatic approaches to the analysis of high-throughput data, such as bisulfite sequencing and Chip-on-chip assays. It features much-desired protocols for plant transgenesis and the analysis of genome stability, with a detailed discussion of their applications to epigenetic studies. Each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a notes section which shares tips on troubleshooting and avoiding known pitfalls.